Application

Anti-Norepinephrine Antibody is an antibody against Norepinephrine for use in IH.

Research CategoryNeuroscience

Research Sub CategoryHormones & Receptors

Immunohistochemistry: 1:200-1:500 with enhanced detection. Glutaraldehyde fixation required for reactivity. Lightly fixed 4% PFA .2-5% glutaraldehyde fixed tissue works well; permeabilization with 0.1-.2% triton X-100 in block ONLY. Dilute antibody solutions in simple buffers without excess proteins or detergents.



Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The chemical compound norepinephrine (NC(8)O(3)H(11), also known as noradrenaline, is a catecholamine that functions as both a neurotransmitter and a hormone. It is released from the adrenal glands and also by certain neurons. It affects parts of the human brain where attention and impulsivity are controlled. This compound affects the fight-or-flight response, activating the sympathetic nervous system to directly increase heart rate, release energy from fat and increase muscle readiness.



The host of physiological changes activated by a stressful event are unleashed in part by activation of a nucleus in the brain stem called the locus ceruleus. This nucleus is the origin of most norepinephrine pathways in the brain. Neurons using norepinephrine as their neurotransmitter project bilaterally from the locus ceruleus along distinct pathways to the cerebral cortex, limbic system, and the spinal cord, among other projections.

Immunogen

Noradrenaline-glutaraldehyde-BSA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid rabbit antiserum with 0.05% sodium azide.

Specificity

Noradrenaline: reactive with noradrenaline reacted with glutaraldehyde; glutaraldehyde required in fixation for reactivity; reactivity with free noradrenaline is very poor.



The cross-reactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.



Compound Cross-reactivity

Noradrenaline-G-BSA 1.0

Octopamine-G-BSA 0.032

Dopamine-G-BSA 0.016

Adrenaline-G-BSA 0.006

Noradrenaline 0.0002

L-Dopa-G-BSA 0.0002

p-Tryamine-G-BSA 0.0002



*The antisera was also tested for specificity using the free-floating PAP technique on rat locus coeruleus.

Abbreviations:

(BSA)= Bovine serum albumin

G)= Glutaraldehyde

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.